Description
The ELISA is a serological method used for detecting antibodies in biological samples. In this procedure, antigens are immobilized on a solid support, such as the bottom of a microtiter plate. The sample containing the antibodies to be tested is then added to the support. If specific antibodies are present in the sample, they bind to the immobilized antigens. After washing away the unbound substances, an enzyme-linked secondary antibody is added, which binds to the primary antibodies. Adding a substrate for the enzyme produces a measurable signal, providing information about the presence and quantity of specific antibodies in the sample.